Deploy python web server on AWS Elastic Beanstalk

I'm deploying an python web server on AWS now and I have a some question about it. I'm using websocket to communicate between back end and front end.

1. Do I have to use framework like django or flask?
2. If not, where should I put the index.html file? in other word, after deploying, how do AWS know the default page of my application?

Thanks in advance.